Specificity of .BETA.-mannanase from Penicillium purpurogenum for konjac glucomannan.

A simple and feasible approach to purify konjac glucomannan from konjac flour--temperature effect.

A simple one-step purification process was provided to extract KGM from KF by phase separation. The results showed that appropriate temperature control was a key factor and the products were inodorous, colourless and of high purity at the optimal temperature 68 °C. In this purification, soluble sugar and starch of extracted KGM were nearly clearly reduced and up to 95%, 80% (T68) of protein and...

Production of ß-glucosidase by Penicillium Purpurogenum

The fungus Penicillium purpurogenum was found to produce intracellular ß-glucosidase. Maximum activity of ß-glucosidase was observed on sucrose. Various cultural parameters of cultivation of P. purpurogenum for production of ß-glucosidase were optimized. Maximum enzyme content was observed after 96 hours of cultivation at 30°C. Addition of amino acids histidine and cysteine induced ß-glucosidas...

Preparation, composition analysis and antioxidant activities of konjac oligo-glucomannan.

Konjac oligo-glucomannan (KOG) was prepared by degradation of konjac glucomannan (KG) using β-mannanase. The hydrolysis process was monitored by the viscosity of the enzymatic hydrolysates. Factors affecting the enzymatic hydrolysis of KG were investigated, and the optimum hydrolysis conditions were as follows: time 2h; temperature 50°C; pH 6.0; and enzymatic concentration 150 U/g. Under these ...

Gelation of Konjac Glucomannan with Different Degree of Acetylation

Carboxymethyl modification of konjac glucomannan affects water binding properties.

The water binding properties of konjac glucomannan (KGM) and carboxymethyl konjac glucomannan (CMKGM) are important for their application in food, pharmaceutical, and chemical engineering fields. The equilibrium moisture content of CMKGM was lower than that of KGM at the relative humidity in the range 30-95% at 25°C. The water absorption and solubility of CMKGM in water solution were lower than...